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目的 以间接免疫酶法观察弓形虫 (RH株 )感染时的急性期病变特点及虫体在主要脏器的动态分布 ,以期为弓形虫病的病理诊断提供依据 ,并对弓形虫致病机理加深理解。方法 用弓形虫 RH株速殖子 10 3个经腹腔感染昆明小鼠 ,于感染后第 2、4、6和 8d,取肝、脾、肺和脑进行间接免疫酶染色。结果 虫体首先在肝脏内被发现 (第 2 d) ,其次是脾和肺 (第 4d) ,最后是脑 (第 8d)。感染早期 ,肝与脾边缘均可见虫体且较多 ;随时间延长 ,肝、脾与肺组织内部逐渐发现少量虫体 ,分布均匀 ;随病程延长 ,各脏器内虫体由少增多 ;脑内发现虫体最晚 ;小鼠体内虫体大量增殖 ,病变严重 ,最终死亡。结论 间接免疫酶染色方法可清楚显示弓形虫感染急性期的组织内弓形虫速殖子及抗原成份。小鼠多脏器被累及如肝、脾、肺和脑。间接免疫酶染色观察结果提示 ,经腹腔感染小鼠时 ,虫体播散途径为 :虫体首先直接侵入腹腔内的器官 ,再通过血液循环播散 ,最后通过血脑屏障到达脑
Objective To observe the characteristics of acute phase lesions and the dynamic distribution of parasites in major organs during the infection of Toxoplasma gondii by indirect immunoenzymatic method, in order to provide a basis for the pathological diagnosis of Toxoplasma gondii and to deepen the pathogenesis of Toxoplasma gondii understanding. Methods Kunming mice were infected with 10 tachyzoites of Toxoplasma gondii RH strain by intraperitoneal injection. Indirect immunoenzymatic staining was performed on the 2nd, 4th, 6th and 8th day after infection in the liver, spleen, lung and brain. Results The parasites were first found in the liver (day 2d), followed by the spleen and lungs (day 4d) and finally to the brain (day 8d). Early infection, the liver and spleen can be seen at the edge of parasites and more; with time, liver, spleen and lung tissue gradually found within a small amount of parasites, evenly distributed; with the duration of the extension, Within the body was found late; large body of mouse parasites multiplication, serious disease, the final death. Conclusion The indirect immunoenzyme staining method can clearly show Toxoplasma gondii tachyzoites and antigenic components in Toxoplasma gondii infection in the acute stage. Mice multiple organs are involved, such as liver, spleen, lung and brain. The results of indirect immunoenzymatic staining suggested that when the mice were infected by intraperitoneal infection, the dissemination route of the parasites was that the parasites directly invade the organs in the abdominal cavity and then spread through the blood circulation, finally reaching the brain through the blood-brain barrier