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目的研究法尼酯X受体(farnesoid X receptor,FXR)的激活在肝细胞中对载脂蛋白F(apolipoprotein F,ApoF)基因表达的影响。方法应用逆转录-聚合酶链反应(RT-PCR)法和Western blot检测不同浓度鹅脱氧胆酸(chenodeoxy cholic acid,CDCA,0、25、50、100μmol/L)和人工合成FXR配体GW4064(0、0.02、0.2、2μmol/L)处理后HepG2和L02细胞中的小分子异二聚体伴侣(small heterodimer partner,SHP)及ApoF的mRNA和蛋白水平变化。结果结果①经FXR天然配体CDCA处理24 h后,不同浓度处理组细胞SHP mRNA水平、ApoF mRNA和蛋白水平均显著高于未处理组(P<0.05)。②经FXR人工合成配体GW4064处理24 h后,不同浓度处理组细胞SHP mRNA水平、ApoF mRNA和蛋白水平均显著高于未处理组(P<0.05)。结论肝细胞株HepG2和L02经FXR配体CDCA或GW4064处理后,FXR被激活,并上调ApoF的mRNA和蛋白水平。
Objective To investigate the effect of farnesoid X receptor (FXR) activation on apolipoprotein F (ApoF) gene expression in hepatocytes. Methods Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot were used to detect the effects of different concentrations of chenodeoxycholic acid (CDCA, 0,25,50,100μmol / L) and synthetic FXR ligand GW4064 0, 0.02, 0.2, 2μmol / L) treatment of HepG2 and L02 cells in small heterodimer partner (small heterodimer partner, SHP) and ApoF mRNA and protein levels. Results ① After treated with CDCA for 24 hours, the levels of ApoF mRNA and protein in SHP mRNA and protein in cells treated with different concentrations of CDCA were significantly higher than those in untreated cells (P <0.05). ② After treated with FXR synthetic ligand GW4064 for 24 h, the levels of SHP mRNA and protein and ApoF mRNA and protein in cells treated with different concentrations were significantly higher than those in untreated cells (P <0.05). Conclusion HepG2 and L02 were activated by FXR ligand CDCA or GW4064 and up-regulated ApoF mRNA and protein levels.