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为了解低氧性肺动脉高压时肺内血管内皮生长因子（ＶＥＧＦ）的改变及其意义，以常压低氧建立大鼠肺动脉高压模型，采用免疫组织化学染色法和Ｅｌｉｓａ法检测模型大鼠肺内ＶＥＧＦ的分布和含量变化。结果显示：低氧３周后大鼠形成明显的肺动脉高压；大鼠肺匀浆内ＶＥＧＦ含量在低氧３周组为４６６．９±７５．５ｐｇ／ｇ，明显高于对照组（３７６．２±４７．１ｐｇ／ｇ）；肺小动脉内膜的ＶＥＧＦ免疫组化染色阳性在低氧大鼠组明显增强。提示低氧在引起肺动脉高压的同时，可强烈刺激ＶＥＧＦ的合成和分泌，后者可能在低氧性肺血管重建过程中发挥重要的作用。
To investigate the changes of pulmonary vascular endothelial growth factor (VEGF) in hypoxic pulmonary hypertension and its significance, the model of pulmonary hypertension in rats was established by normobaric hypoxia. Immunohistochemistry and Elisa method were used to detect the pulmonary VEGF distribution and content changes. The results showed that the pulmonary hypertension was formed in rats after hypoxia for 3 weeks. The content of VEGF in lung homogenate was 466.9 ± 75.5 pg / g in hypoxia 3 weeks group, which was significantly higher than that in control group (376.2 ± 47.1pg / g). Immunohistochemical staining of VEGF in the intima of pulmonary arterioles was significantly increased in hypoxia rats. It is suggested that hypoxia may stimulate the synthesis and secretion of VEGF while causing pulmonary hypertension, which may play an important role in hypoxic pulmonary vascular remodeling.