论文部分内容阅读
脐带间充质干细胞因其来源广泛,易扩增,低免疫原性等特点被人们越来越多地应用于疾病治疗的研究中。本研究旨在为重症肌无力疾病探求一种新的应用干细胞的治疗方法。将脐带间充质干细胞(UCMSC)移植到重症肌无力大鼠中,应用免疫荧光法观察人源化细胞的分布,ELISPOT法观察MSC对B细胞原位分泌抗体的影响,Transwell试验检测分泌IFN-γ的水平。结果发现,经静脉输注的UCMSC能快速的迁移到炎症部位和局部淋巴结,而且在淋巴结的髓质区也可以检测到人源化的细胞。体外原位检测乙酰胆碱受体(AchR)抗体分泌的试验发现,当MSC与淋巴结来源的淋巴细胞充分接触,能有效地抑制AchR抗体的产生。Transwell试验显示,UCMSC与CD4T细胞直接接触,能有效地降低IFN-γ的产生,在一定程度上缓解重症肌无力体内的Th1/Th2细胞失衡的免疫状态。结论 :在重症肌无力大鼠中,MSC可能通过相互接触或/和释放细胞因子而调节机体的免疫反应,这为重症肌无力提供了一种潜在的治疗新途径。
Umbilical cord mesenchymal stem cells are widely used in the research of disease treatment because of their wide source, easy amplification, low immunogenicity and so on. The aim of this study is to explore a new therapeutic approach to stem cell therapy for myasthenia gravis disease. Human umbilical cord mesenchymal stem cells (UCMSCs) were transplanted into myasthenia gravis rats. Immunofluorescence staining was used to observe the distribution of humanized cells. ELISPOT method was used to observe the effect of MSC on the secretion of B cells in situ. Transwell assay was used to detect IFN- γ level. The results showed that UCMSCs transfused rapidly to the site of inflammation and local lymph nodes, but also humanized cells can be detected in the medullary region of lymph nodes. In situ detection of AchR antibody secretion in vitro found that MSC and lymph node-derived lymphocytes in full contact, can effectively inhibit AchR antibody production. Transwell test showed that direct contact of UCMSC with CD4 T cells can effectively reduce the production of IFN-γ and relieve the imbalance of Th1 / Th2 cell immune status to some extent. Conclusion: In myasthenia gravis rats, MSC may regulate the body’s immune response by contacting and / or releasing cytokines with each other, which provides a potential new therapeutic approach for myasthenia gravis.