目的:建立天舒胶囊中川芎嗪的含量测定方法。方法:用甲醇提取,以Waters Nova-pak C_(18)色谱柱(3.9 mm×300mm,4μm)为固定相,甲醇-水(50:50 V/V)为流动相,检测波长为282nm。结果:川芎嗪在10～50μg·ml~(-1)范围内呈良好的线性关系,r=0.9998;平均回收率为99.3％,RSD=1.6％;连续进样的精密度是RSD=0.8％。结论:方法能消除其它成分的干扰,准确可靠、重现性好,操作简便快速。 Objective: To establish a method for the determination of tetramethylpyrazine in Tianshu capsule. Methods: The extract was extracted with methanol. The stationary phase was Waters Nova-pak C18 column (3.9 mm×300 mm, 4 μm). The mobile phase consisted of methanol-water (50:50 V/V) and the detection wavelength was 282 nm. RESULTS: There was a good linear relationship between ligustrazine in the range of 10-50 μg·ml -1 , r=0.9998; the average recovery was 99.3%, RSD was 1.6%; the precision of continuous injection was RSD=0.8% . Conclusion: The method can eliminate the interference of other components, accurate and reliable, good reproducibility, and simple and fast operation.