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本文提出了双孢菇凝集素（ＡｇａｒｉｃｕｓＢｉｓｐｏｒｕｓＡｇｇｌｕｔｉｎｉｎ简称ＡＢＡ）的制备及性质研究。采用稀盐溶液浸提、酒精分级沉淀、ＤＥ—２３和ＣＭ－２３柱层析分离纯化，得Ａ、Ｂ两大组分，经ＰＡＧ检测组分Ａ为三条带，组分Ｂ为两条带。ＳＤＳ－ＰＡＧＥ测定表观分子量：组分Ａ三条还分别为８２０００、６１０００、５１０００；组分Ｂ两条带分别为２３０００、２２０００。由ＩＦＥ测得等电点：Ａ组分ＰＩ分别为ｐＨ５．０６、ｐＨ５．４６、ｐＨ６．０１；组分Ｂ等电点ＰＩ分别为ｐＨ６．２５和ｐＨ７．６２。Ａ、Ｂ两大组分凝血活性单位；Ａ组分为２－１３ｍｇ／ｍｌ，Ｂ组分为２－６ｍｇ／ｍｌ。
In this paper, the preparation and properties of AgaricusBisporusAgglutinin (ABA) were studied. Using diluted salt solution leaching, alcohol fractionation precipitation, DE-23 and CM-23 column chromatography separation and purification, the A, B two components, the PAG test component A is three, component B is two . The apparent molecular weight was determined by SDS-PAGE. The three components of component A were also 82000, 61000, and 51000 respectively. The two components of component B were 23000 and 22000 respectively. The isoelectric point measured by IFE: A component PI were pH5.06, pH5.46, pH6.01; component B isoelectric point PI were pH6.25 and pH7.62. A, B two components coagulation activity unit; A component is 2-13mg / ml, B component is 2-6mg / ml.