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目的 :阐明烧伤休克时 beta-2整合素与白细胞 -内皮细胞粘附间的关系。方法 :将 16只 SD大鼠随机均分为对照组和烧伤休克组。应用流式细胞技术检测中性粒细胞及单细胞表面 beta-2整合素CD11a( LFA-1)和 CD11b( Mac-1)的表达量变化 ,同时比较观察二组动物肠系膜微循环中微静脉内白细胞与内皮细胞粘附数量变化。结果 :二组自身对照 (烫伤前与烫伤后 3 h或开腹前与开腹后 3 h)及相互对比结果显示 ,中性粒细胞及单核细胞表面 CD11a表达量均无显著差异 ( P>0 .0 5 ) ;在烧伤组 ,单核细胞 CD11b有显著降低( P<0 .0 5 ) ,而中性粒细胞 CD11b有显著升高 ( P<0 .0 5 )。微循环观察结果显示 ,休克组动物烫伤后随时程延长附壁滚动及紧密粘附的白细胞数量明显增多 ,而对照组则无明显变化。结论 :烧伤休克状态下 ,白细胞表面 CD11a数量无显著变化 ,而 CD11b数量则有所改变。白细胞 -内皮细胞粘附力的增强可能有 beta-2整合素功能活性上调参与。
Objective: To elucidate the relationship between beta-2 integrin and leukocyte-endothelial cell adhesion in burn shock. Methods: Sixteen SD rats were randomly divided into control group and burn shock group. The changes of expression of beta-2 integrin CD11a (LFA-1) and CD11b (Mac-1) on neutrophils and single cells were detected by flow cytometry. Leukocyte and endothelial cell adhesion number changes. Results: The results of self-control (pre-scald and post-scald 3 h or preoperation and 3 h postoperation) and the contrast between the two groups showed no significant difference in the expression of CD11a on neutrophils and monocytes (P> 0.05). In the burn group, monocyte CD11b was significantly decreased (P <0.05) and neutrophil CD11b was significantly increased (P <0.05). The results of microcirculation showed that the numbers of white blood cells in the shock group prolonged after scalding and adhered to the scaffold significantly increased, while the control group showed no significant changes. Conclusion: In burn shock state, there is no significant change in the number of CD11a on the surface of leucocytes, while the number of CD11b is changed. Increased leukocyte-endothelial cell adhesion may involve increased beta-2 integrin functional activity.