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目的:建立离子对RP-HPLC法测定五维赖氨酸颗粒中烟酰胺、维生素B6、维生素B2、维生素B1的含量。方法:用Zorbax Eclipe XDB-C18柱(250 mm×4．6 mm,5μm),流动相为0．005 mol·L-1庚烷磺酸钠溶液(含0．5％冰醋酸和0．05％三乙胺)-甲醇(72:28)为流动相,流速为1．0 ml·min-1,检测波长为280 nm。结果:烟酰胺、维生素B6、维生素B2及维生素B1的线性范围分别为0．32-15．77μg(r=0．999 7),0．05～2．61μg(r=0．999 9)、0．05-2．65μg(r=0．999 9)及0．45-22．37μg(r= 0．999 9),平均回收率分别为98．3％(RSD 1．3％,n=9)、101．1％(RSD 1．9％,n=9)、99．3％(RSD 1．0％,n=9)、98．9％(RSD 1．1％,n=9)。结论:本法简便,快速,可用于五维赖氨酸颗粒产品的质量控制。
Objective: To establish an RP-HPLC method for the determination of nicotinamide, vitamin B6, vitamin B2 and vitamin B1 in five-dimensional lysine granules. METHODS: A Zorbax Eclipe XDB-C18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase was 0.005 mol·L -1 sodium heptane sulfonate solution (containing 0.5% glacial acetic acid and 0.05 % Triethylamine) -methanol (72:28) as the mobile phase with a flow rate of 1.0 ml · min-1 and a detection wavelength of 280 nm. Results: The linear ranges of niacinamide, vitamin B6, vitamin B2 and vitamin B1 were 0.32-15.77μg (r = 0.999 7), 0.05-2.61μg (r = 0.999 9), 0.05-2.65μg (r = 0.999 9) and 0.45-22.37μg (r = 0.999 9), the average recoveries were 98.3% (RSD 1.3%, n = 9), 101.1% (RSD 1.9%, n = 9), 99.3% (RSD 1.0%, n = 9), 98.9% (RSD 1.1%, n = 9) . Conclusion: This method is simple, rapid and can be used for quality control of five-dimensional lysine granules.