定量分析皂甙是一个长期以来的分析化学问题,已用于皂甙分析的 GC 方法局限于仅能分离和定量皂甙的甙元部分,这样既损失了分子中糖甙部分的结构信息,又可能在测定的处理过程中损失皂甙。本文研究了分离和定量窄叶羽扇豆 Lupinus angustifolius种子中的原型皂甙的 HPLC 方法,并与 GC测定结果做了比较。 Quantitative analysis of saponins is a long-standing problem in analytical chemistry. The GC method that has been used for saponin analysis is limited to the ability to separate and quantify only the glycogen moiety of saponins, which loses both structural information of the glycosyl moieties in the molecule and may be measured. Loss of saponins during processing. This paper studied the HPLC method for the separation and quantification of the prototypical saponins from the seeds of Lupinus angustifolius, and compared it with the GC results.