论文部分内容阅读
大鼠10％、30％体表面积Ⅲ°烫伤4h后,测定血浆、红细胞、肝和肺匀浆上清液的MDA含量,全血、肝和肺匀浆上清液的SOD、GSSG-Px和CAT活性,以及肝匀浆上清液的XO活性。结果表明,烫伤大鼠伤后组织MDA含量和SOD活性,以及肝中XO、CAT活性,肺和全血中GSSG-Px活性均显著增加,除肝和肺中SOD活性以及全血中GSSG-Px活性100％与30％烫伤组间无显著差异外,其余均与烫伤面积相关;而全血和肺中CAT活性伤后降低,肝中GSSG-Px活性10％和30％烫伤组分别高于和低于对照组。本实验结果显示,体表烫伤面积较小(10％)时,机体可能通过诱导合成主要的抗氧化酶,提高抗氧化能力,以对抗自由基、LPO的损伤,而当体表烫伤面积较大(30％)时,不同组织抗氧化酶对氧应激的反应有所不同。
The content of MDA in plasma, erythrocyte, liver and lung homogenate supernatant were determined after 10% of rat and 30% of body surface area Ⅲ of scald for 4 hours. SOD, GSSG-Px, CAT activity, and XO activity of liver homogenate supernatant. The results showed that the content of MDA and the activity of SOD, the activities of XO and CAT in the liver and the activity of GSSG-Px in lung and whole blood were significantly increased in scalded rats, the activity of SOD in liver and lung and GSSG-Px in whole blood Activity of 100% and 30% scald group no significant difference, the rest are related to the scalded area; and whole blood and lung activity of CAT decreased after injury, liver GSSG-Px activity 10% and 30% scald group were higher than and Lower than the control group. The results of this study showed that when body surface burn area is small (10%), the body may induce the synthesis of major anti-oxidant enzymes and improve the anti-oxidant ability to combat the free radical and LPO damage. However, when body surface burn area is relatively large (30%), different organizations antioxidant enzymes respond differently to oxygen stress.