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目的借鉴血清药物化学的方法,探讨川芎效应组分在血液及靶组织脑脊液中的移行成分(即药物以原型进入到体液的化学成分),从而为快速、有效地确定川芎效应组分的物质基础,进一步建立其完善的质量标准奠定基础。方法通过HPLC指纹图谱和紫外光谱分析,比较空白血清、含药血清,空白脑脊液、含药脑脊液成分异同,确定川芎效应组分的血中移行成分与脑脊液移行成分,并采用HPLC-DAD-MS/MSn分析技术推测移行成分的化学结构。结果阿魏酸、洋川芎内酯Ⅰ为川芎效应组分的血清移行成分,洋川芎内酯Ⅰ为其脑脊液移行成分,即洋川芎内酯Ⅰ为川芎效应组分即入血又入脑的化学成分。结论洋川芎内酯Ⅰ很可能是川芎效应组分治疗偏头痛最重要的生物活性成分。
OBJECTIVE: To study the chemical composition of Ligusticum chuanxionggensis in cerebrospinal fluid of the blood and target tissues (ie, the chemical composition of the drug entering the bodily fluid by prototype), so as to determine the material basis of the components of Ligusticum chuanxiong to be effective quickly and effectively , To further establish its perfect quality standards and lay the foundation. Methods HPLC fingerprint and ultraviolet spectrum analysis were used to compare the components of blood circulation and cerebrospinal fluid in the components of the effects of the components of Rhizoma Ligustici on the blood components of cerebrospinal fluid in serum, MSn analytical techniques speculate on the chemical composition of migratory components. Results Ferulic acid and Rhizoma Chuanxiong Lactone Ⅰ were the serum components of Chuanxiong Rhizoma Chuanxiong and its components, and Yang Chuanxiong Lactone Ⅰ was the component of cerebrospinal fluid migration, that is, Chuanxiong Lactone Ⅰ was the component of Rhizoma Chuanxiong, ingredient. Conclusion Yang Chuanxiong Lactone Ⅰ is likely to be the most important biologically active component of migraine induced by Rhizoma Chuanxiong.