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目的观察三氯化铝 (AlCl3)对染毒大鼠尿中单胺类神经递质含量变化的影响 ,寻找早期生物学监测指标 ,为探讨铝的神经毒性作用机制提供依据。方法144只Wistar大鼠随机分为注射剂量分别为5.0、15.0、25.0mg/Kg的3个剂量组和生理盐水对照组。用原子吸收分光光度计测定尿铝含量 ,用高效液相色谱仪测定尿中高香草酸 (HVA)及香草扁桃酸 (VMA)含量。结果各时间剂量组大鼠尿铝均显著高于对照组 (P<0.01);尿中的HVA和VMA水平均较高 ,各时间剂量组VMA水平显著高于对照组 (P<0.01) ;25mg/kg组HVA也显著高于对照组 (P<0.05)。结论铝可影响单胺类神经递质代谢 ,尿铝和VMA可用作铝引起的中枢神经系统功能异常的早期生物学监测指标
Objective To investigate the effect of aluminum trichloride (AlCl3) on the changes of monoamine neurotransmitters in urine of rats exposed to AlCl3, and to find out the early biological indicators for monitoring the neurotoxic effect of aluminum. Methods One hundred and forty-four Wistar rats were randomly divided into 3 dose groups (5.0, 15.0, 25.0 mg / Kg) and saline control group. The contents of aluminum and aluminum were determined by atomic absorption spectrophotometer. The contents of HVA and VMA in urine were determined by high performance liquid chromatography. Results The urinary contents of aluminum and aluminum in rats in each time-dose group were significantly higher than those in the control group (P <0.01). The levels of HVA and VMA in urine were higher than those in the control group (P <0.01) / kg group HVA was significantly higher than the control group (P <0.05). Conclusion Aluminum can affect monoamine neurotransmitter metabolism. Urinary aluminum and VMA can be used as early biological indicators of central nervous system dysfunction caused by aluminum