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应用ＬＳＡＢ免疫组化方法检测了８２例原发性肺癌组织标本Ｐ~（５３）蛋白及增殖细胞核抗原（ＰＣＮＡ）的表达．结果显示肺癌Ｐ~（５３）蛋白表达与ＰＣＮＡ表达间呈显著正相关（Ｐ＜０．０１），Ｐ~（５３）蛋白阳性表达者ＰＣＮＡ分级显著高于Ｐ~（５３）蛋白阴性表达者；Ｐ~（５３）蛋白及ＰＣＮＡ的表达均与肺癌组织学分级关系不显著（Ｐ＞０．０５）．在肺腺癌有无淋巴结转移两组间Ｐ~（５３）蛋白及ＰＣＮＡ表达均有显著性差异且与患者的预后有关（Ｐ＜０．０５），提示两者的检测对于评估肺腺癌患者的预后有重要意义；在肺鳞癌，ＰＣＮＡ表达与患者的预后有关（Ｐ＜０．０５），而Ｐ~（５３）蛋白表达与患者预后的关系不显著（Ｐ＞０．０５）．
The expression of P53 protein and proliferating cell nuclear antigen (PCNA) in 82 specimens of primary lung cancer were detected by LSAB immunohistochemistry. The results showed that there was a significant positive correlation between the expression of P53 protein and the expression of PCNA in lung cancer (P<0.01). The PCNA grade of P53 protein positive expression was significantly higher than that of P53 protein negative expression. The expression of P53 protein and PCNA were not significantly associated with the histological grade of lung cancer (P>0.05). The expression of P53 protein and PCNA in the lung adenocarcinoma with or without lymph node metastasis were significantly different and correlated with the prognosis of patients (P<0.05), suggesting that the detection of both of them is useful for the evaluation of lung adenocarcinoma patients. The prognosis is significant; in lung squamous cell carcinoma, the expression of PCNA is related to the prognosis of patients (P<0.05), while the expression of P53 protein has no significant relationship with the prognosis of patients (P>0.05).