论文部分内容阅读
目的建立同时测定“麦味参”(按人参-麦冬-五味子为1∶3∶1配伍)中20种活性成分的HPLC方法。方法采用色谱柱(150 mm×4.6 mm,5μm),乙腈(A)-水(B)为流动相,梯度洗脱,体积流量为1.0 mL/min,检测波长203 nm,柱温35℃。结果人参皂苷Rg_1、Re、Rf、Rb_1、Rg_2、Rc、Rb_2、Rb_3、Rh_2、F_1、Rd、F_2、Rg_3,原人参三醇,compound K,原人参二醇,3种五味子木脂素五味子醇甲、五味子甲素、五味子乙素和麦冬皂苷D均得到良好的线性关系(r≥0.999 6),平均回收率均在96%~102%,RSD均小于2%。结论方法准确可靠,灵敏度高,专属性强,结果稳定,重复性好,可用作“麦味参”的多成分质量控制。
OBJECTIVE To establish an HPLC method for the simultaneous determination of 20 active ingredients in “Atractylodes macrocephalae” (a 1: 3: 1 combination of Panax ginseng, Radix Ophiopogonis and Fructus Schisandrae). Methods The column (150 mm × 4.6 mm, 5 μm) and acetonitrile (A) - water (B) were used as the mobile phase and eluted with a gradient of 1.0 mL / min. The detection wavelength was set at 203 nm and the column temperature was 35 ℃. Results Ginsenosides Rg_1, Re, Rf, Rb_1, Rg_2, Rc, Rb_2, Rb_3, Rh_2, F_1, Rd, F_2, Rg_3, Protopanaxol, compound K, Protopanaxadiol, Schisandra chinensis A, Schisandrin, Schisandrin B and Ophiopogon japonicus D both got a good linear relationship (r≥0.9996), the average recoveries ranged from 96% to 102% with RSD less than 2%. Conclusion The method is accurate, reliable, sensitive and specific, with stable results and good reproducibility. It can be used as a multi-component quality control method for “Atractylodes macrocephala”.