目的:探讨家族聚集性大动脉粥样硬化型(large-artery atherosclerosis，LAA)脑梗死患者与散发性LAA脑梗死患者环氧化酶(cyclooxygenase，COX)基因表达差异。方法:收集25例家族聚集性LAA脑梗死和散发性LAA脑梗死患者，另外选择25例健康人群作为对照组。检测COX-1及COX-2基因相对表达量。结果:COX-1基因在家族聚集性LAA脑梗死组、散发性LAA脑梗死组、对照组被试表达分别为0.5436±0.2737、0.2400±0.1656、0.8340±0.3799。COX-1基因在家族聚集性LAA脑梗死组和散发性LAA脑梗死组表达下调，与对照组比较差异有统计学意义(均n P<0.05)。COX-2基因分别在家族聚集性LAA脑梗死组、散发性LAA脑梗死组、对照组表达为1.3672±0.8249，1.3932±0.7158，0.7212±0.9623。COX-2基因在家族聚集性LAA组和散发性LAA组表达上调，与对照组比较均差异有统计学意义(均n P0.05)。n 结论:家族聚集性LAA脑梗死患者与散发性LAA脑梗死患者COX-1基因表达存在明显差异。“,”Objective:To investigate the differential expression of cyclooxygenase (COX) gene between familial aggregated atherosclerotic large-artery atherosclerosis(LAA) cerebral infarction patients and sporadic LAA cerebral infarction patients.Methods:Twenty-five patients with familial aggregation LAA cerebral infarction (familial aggregation LAA group) and 25 sporadic LAA cerebral infarction patients (sporadic LAA group) were collected.Another 25 patients with non-cardiovascular and cerebrovascular diseases were selected as control group.Real-time quantitative PCR and ELISA were used to detect COX-1 and COX-2 protein in the supernatant of samples.Results:The expression level of COX-1 gene was 0.5436±0.2737, 0.2400±0.1656 and 0.8340±0.3799 in the familial aggregation, sporadic LAA cerebral infarction and control groups.Compared with control group, COX-1 gene expression in sporadic LAA cerebral infarction group and familial aggregation LAA cerebral infarction group was significantly down-regulated, the differences were significant(n P<0.05). The expression level of COX-2 gene was 1.3672±0.8249, 1.3932±0.7158 and 0.7212±0.9623 in the familial aggregation, sporadic LAA cerebral infarction and control groups.Compared with the normal control group, the expression of COX-2 gene in familial aggregation LAA cerebral infarction group and sporadic LAA cerebral infarction group increased significantly(n P0.05).n Conclusion:There is significant difference in COX-1 gene expression between patients with familial LAA and sporadic LAA cerebral infarction.