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为研究A型GABA受体α1亚型(GABRA1)在癫痫患者病灶额叶及颞叶脑组织中的表达变化与癫痫发病机制的关系,采用TaqMan探针荧光定量PCR检测GABRA1在癫痫患者病灶额叶和颞叶脑组织及对照组额叶和颞叶脑组织中的表达量的差异.结果表明,对照组及癫痫患者额叶GABRA1平均相对表达强度为3.785±1.444和4.399±2.89,两者间无显著差异(P>0.05).对照组颞叶脑组织中GABRA1平均表达量是癫痫患者的4.5倍,对照组及癫痫患者颞叶GABRA1平均相对表达强度为26.802±8.983和5.986±5.07,结果具有统计学意义(P<0.05).提示,难治性癫痫患者GABRA1表达在不同组织中的改变并非单一性改变,GABRA1表达量的改变导致癫痫的发生存在组织特异性;GABRA1在颞叶脑组织中的表达减少与癫痫的发生,尤其是难治性癫痫的发生有密切的关系,可能是难治性癫痫致病的因素之一.该研究结果为进一步研究癫痫的发病机制及防治提供了科学依据.
To investigate the relationship between the expression of GABA1 type 1 GABA1 and the pathogenesis of epilepsy in frontal lobe and temporal lobe of epileptic patients, TaqMan probe real-time quantitative PCR was used to detect GABRA1 in frontal lobe of epileptic patients And temporal lobe brain tissue and the control group.The results showed that the average relative expression intensity of frontal lobe GABRA1 in control group and epileptic patients was 3.785 ± 1.444 and 4.399 ± 2.89, (P> 0.05) .In the control group, the average expression of GABRA1 in temporal lobe brain tissue was 4.5 times that of epilepsy patients, and the average relative expression intensity of GABRA1 in temporal lobe of control group and epileptic patients was 26.802 ± 8.983 and 5.986 ± 5.07, respectively (P <0.05), suggesting that in patients with refractory epilepsy GABRA1 expression changes in different tissues is not a single change in the expression of GABRA1 changes in the presence of tissue-specific epilepsy; GABRA1 in temporal lobe brain tissue Decreased expression and the occurrence of epilepsy, especially refractory epilepsy is closely related to the occurrence of refractory epilepsy may be one of the factors.The results of the study for the further study of epilepsy pathogenesis and prevention and treatment For a scientific basis.