论文部分内容阅读
复杂混合物样品分高应采用统一方法、多柱系统。多元切换或多柱串联调节选择性在仪器和操作上均不适合作为通用方法。因此提出了智能多柱系统的概念，探讨了智能多柱系统选择性优化原则和多柱系统选择性优化方法。对多种官能团取代基化合物混合物样品，最佳多柱系统是针对其中的特殊重叠组分进行的，一般采用选择性有足够差别的弱极性柱子组成最佳多柱系统。对同族化合物异构体复杂混合物，多拄系统一般采用作用距离和分子构型差别大的柱子组成多柱系统，对光学异构体则必须采用相应的手性柱子组成多柱系统。双柱规律保留值方程中Ａ系数可以定量比较固定相间对异构体选择性的相对变化，Ｃ值则可以用于描述不同族化合物混合物分离时选择性匹配程度。用实际样品分析数据和例子验证了方法的正确性。
Complicated mixture sample points should be a uniform method, multi-column system. Multivariate switching or multi-column series adjustment selectivity is not suitable as a general method both in instrumentation and operation. Therefore, the concept of intelligent multi-column system is proposed, and the principle of selective optimization of multi-column system and selective optimization of multi-column system are discussed. For multiple functional groups of substituted compound mixture samples, the best multi-column system is for one of the special overlapping components and the best multi-column system is generally composed of weakly polar columns of sufficiently selective selectivity. For complex mixtures of isomeric compounds of the same family, multi-column systems typically employ multiple column systems with columns of widely varying distance and molecular configuration, and the corresponding optical isomers must be composed of multiple column systems with corresponding chiral columns. The A-factor in the two-column retention equation can be used to quantitatively compare the relative changes in the isomer selectivity between fixed phases. The C-value can be used to describe the degree of selectivity for different mixtures of compounds. The actual sample analysis data and examples verify the correctness of the method.