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目的探讨哮喘患者急性期诱导痰液中嗜酸性粒细胞(EOS)、嗜酸性细胞阳离子蛋白(ECP)及血清白细胞介素-13(IL-13)的水平变化及在哮喘发病机制中的作用。方法选择55例支气管哮喘急性期患者和20例健康对照者的痰液,行EOS计数并测定ECP水平。同时采用固相酶联免疫吸附试验(ELISA)法检测血清中IL-13的水平,并对结果进行统计学处理。结果支气管哮喘急性期患者诱导痰液中EOS、ECP分别与血清IL-13呈正相关,且诱导痰液中EOS、ECP及血清IL-13的水平均明显高于健康对照组(P<0.05)。结论嗜酸性粒细胞是导致哮喘的主要炎性细胞,而IL-13又是嗜酸性粒细胞激动剂并参与支气管哮喘发病的病理生理过程,诱导痰液EOS、ECP和血清IL-13检测是哮喘诊断及病情转归的一个重要实验室指标。
Objective To investigate the changes of eosinophil (EOS), eosinophil cationic protein (ECP) and serum interleukin-13 (IL-13) levels in asthmatic patients induced sputum and their roles in the pathogenesis of asthma. Methods Fifty-five sputum samples from patients with acute bronchial asthma and 20 healthy controls were selected for EOS counting and ECP levels were measured. At the same time, the level of IL-13 in serum was detected by solid phase enzyme-linked immunosorbent assay (ELISA), and the results were statistically analyzed. Results The levels of EOS and ECP in induced sputum of patients with bronchial asthma were positively correlated with serum IL-13, and the levels of EOS, ECP and serum IL-13 in induced sputum were significantly higher than those in healthy controls (P <0.05). Conclusion Eosinophils are the major inflammatory cells that cause asthma. IL-13 is also an eosinophil agonist and participates in the pathophysiological process of bronchial asthma. Induced sputum EOS, ECP and serum IL-13 are asthma Diagnosis and prognosis of an important laboratory indicators.