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This work demonstrated quantitatively that DSA(dansylamide) and apo CA(dezincified carbon anhydrase) interacted with each other in the presence of Zn2+(zinc ion). The fl uorescence emission of DSA in the presence of Zn2+ and the intensity of fl uorescence, which was proportional to the concentration of Zn2+, could be used in the measurement of Zn2+ concentration. Considering that Zn2+ was the active center of enzymes like CA(carbon anhydrase), two dezincifi cation reagents were compared to ensure the validity of our method. A certain range concentration of Zn2+ could be measured by the reaction product of apo CA, DSA and Zn2+, where the measurement limitation was about 60 nmol/L. Interaction of CA with DSA was also studied with infrared spectrometry.
This work demonstrates a quantitatively that DSA (dansylamide) and apo CA (dezincified carbon anhydrase) interacted with each other in the presence of Zn2 + (zinc ion). The fl uorescence emission of DSA in the presence of Zn2 + and the intensity of fl uorescence, which was proportional to the concentration of Zn2 + concentration. Zn2 + was the active center of enzymes like range concentration of Zn2 + could be measured by the reaction product of apo CA, DSA and Zn2 +, where the measurement limitation was about 60 nmol / L. Interaction of CA with DSA was also studied with infrared spectrometry.