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该文研究窖蛋白(Caveolin-1)对乳腺癌细胞系MCF-7细胞增殖与存活的影响。运用蛋白质印迹方法(Western blot)检测发现,caveolin-1在5株不同细胞系均只有低表达。运用电穿孔转染方法在乳腺癌细胞系中高表达Caveolin-1,运用Western blot检测转染后Caveolin-1表达情况发现,转染后细胞内Caveolin-1表达上升,并具有生物活性。运用单核细胞直接细胞毒性测定法(MTT)检测发现,转染后乳腺癌细胞系MCF-7增殖速度降低。运用Western blot方法和免疫荧光(immunofluorescence)方法检测转染后细胞凋亡途径的变化,磷酸化的P38蛋白含量上升,Bax表达量明显上升。据此推测Caveolin-1抑制MCF-7细胞的增殖和存活,并诱导基于Bax途径的细胞凋亡。
This article studies the effect of caveolin-1 on the proliferation and survival of breast cancer cell line MCF-7. Western blot analysis showed that caveolin-1 was only down-regulated in five different cell lines. The expression of Caveolin-1 was highly expressed in breast cancer cell lines by electroporation. The expression of Caveolin-1 after transfection was detected by Western blot. The expression of Caveolin-1 in the cell line was up-regulated after transfection and had biological activity. Using monocytogenetic direct cytotoxicity assay (MTT), we found that the proliferation rate of MCF-7 cells was decreased after transfection. Western blot and immunofluorescence were used to detect the changes of apoptotic pathway after transfection. The phosphorylated P38 protein content and the expression of Bax increased significantly. It is speculated that Caveolin-1 inhibits the proliferation and survival of MCF-7 cells and induces apoptosis based on the Bax pathway.