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目的:建立氰尿酸Wistar大鼠泌尿系结石模型,为预防和治疗氰尿酸结石提供实验依据。方法:把72只Wistar雄性大鼠随机分成对照组36只和氰尿酸灌胃组36只。其中灌胃组根据不同灌胃剂量又分成A(50mg·kg-1·d-1)、B(100mg·kg-1·d-1)、C(300mg·kg-1·d-1)三个亚组,每亚组各12只。每天所给氰尿酸剂量加入日需1/3饮水中,分两次于每天上午、下午灌胃,其余所需饮水晚上供给;对照组灌胃方法同实验组,但饮水中不加入药物。观察指标:厌食、嗜睡、呕吐、血尿、少尿或无尿。大鼠灌胃过程中出现上述症状中任何一项后采用吸入二氧化碳处死;有一半大鼠达到灌胃结束指标后全部处死,观察大鼠泌尿系结石情况。结果:灌胃第30天,A组2只、B组10只、C组12只大鼠出现厌食、活动减少、血尿等情况,大鼠处死后用福尔马林固定,病理检查见A组1只、B组4只、C组7只大鼠肾小管和集合管的结石样物质,肾小管、集合管扩张、上皮细胞肿胀变性;对照组大鼠未见异常。结论:氰尿酸灌胃Wistar大鼠30天,在大鼠肾脏集合管和肾小管形成结晶样物质,导致肾小管和集合管积水扩张,并且随着摄入量的增加形成结石的速度加快,成功建立了氰尿酸大鼠泌尿系结石模型,对氰尿酸泌尿系结石的预防和治疗提供了实验依据。
Objective: To establish a urinary calculi model of cyanuric Wistar rats and provide experimental evidence for the prevention and treatment of cyanuric acid stones. Methods: Seventy two Wistar male rats were randomly divided into control group (36) and cyanuric acid group (36). The gavage group was divided into A (50mg · kg-1 · d-1), B (100mg · kg-1 · d-1) and C (300mg · kg-1 · d-1) A subgroup, each subgroup of 12. Cyanuric acid daily dose to be added to the day to drink 1/3 of water, two times in the morning every morning, afternoon gavage, the rest of the supply of drinking water supply; control group gavage method with the experimental group, but without adding drugs in the drinking water. Observations: Anorexia, lethargy, vomiting, hematuria, oliguria or anuria. Rats during the gavage of any of the above symptoms after the use of carbon dioxide inhalation; half of the rats reached the end of gavage indicators were sacrificed to observe the urinary stones in rats. Results: On the 30th day after gavage, two rats in group A, 10 rats in group B and 12 rats in group C appeared anorexia, activity reduction and hematuria. After the rats were sacrificed, they were fixed with formalin. The pathological examination was performed in group A 1 in group B, 4 in group B and 7 in group C. The stone-like substances of renal tubules and collecting tubes in tubs of rats in group C were dilated. The swelling and degeneration of epithelial cells were observed. No abnormality was found in the control group. CONCLUSION: Cyanuric acid instilled Wistar rats for 30 days to form crystal-like substance in the renal tubules and tubules in rats, resulting in the expansion of tubules and collecting ducts. With the increase of intake, the formation of stones is accelerated, Successfully established a model of urinary calculus in rats with cyanuric acid and provided experimental evidence for the prevention and treatment of cyanuric acid urinary stones.