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目的:对补阳还五汤及总苷元在大鼠血浆中阿魏酸的含量进行测定,并研究其药物动力学。方法:采用反相高效液相色谱法,色谱柱:C_(18)柱(250 mm×4.6 mm,5μm),检测波长:322 nm,流动相:甲醇-水-冰醋酸(35:65:0.47),流速:1 ml·min~(-1),柱压:(2200±60)psi;温度:35℃。药物动力学参数采用3P87软件处理。结果:血浆样品中阿魏酸线性范围为1.01～5.05 ng(r=0.999 8),平均方法回收率为98.85%,RSD为1.3%。补阳还五汤中阿魏酸的t_(1/2α)为1.31 min;t_(1/2β)为58.9 min;CL为(2.83±0.12)ml·min~(-1)·kg~(-1);AUC为(531.6±67.9)μg·min·ml~(-1);总苷元中阿魏酸的t_(1/2α)为1.39 min;t_(1/2β)为52.3 min;CL为(3.43±0.22)ml·min~(-1)·kg~(-1);AUC为(593.3±45.7)μg·min·ml~(-1),两组药动学参数中B(μg·ml~(-1))、t_(1/2)β(min)和V_c(ml·kg~(-1))存在显著差异(P<0.05)。结论:补阳还五汤复方及总苷元中阿魏酸药物动力学为二室模型。中药复方成分组合对药物动力学参数有一定影响。
Objective: To determine the contents of ferulic acid in Buyang Huanwu Decoction and total aglycone in rat plasma and to study its pharmacokinetics. METHODS: A reversed-phase high performance liquid chromatography method was used. Column: C 18 column (250 mm×4.6 mm, 5 μm), detection wavelength: 322 nm, mobile phase: methanol-water-glacial acetic acid (35:65:0.47) ), flow rate: 1 ml·min~(-1), column pressure: (2200±60) psi; temperature: 35°C. Pharmacokinetic parameters were processed using 3P87 software. Results: The linear range of ferulic acid in plasma samples was 1.01 to 5.05 ng (r=0.999 8). The average method recovery was 98.85% and the RSD was 1.3%. The t_(1/2α) of ferulic acid in Buyang Huanwu Decoction was 1.31 min; t_(1/2β) was 58.9 min; CL was (2.83±0.12) ml·min~(-1)·kg~(- 1); AUC was (531.6±67.9) μg·min·ml -1; t_(1/2α) of ferulic acid in total aglycone was 1.39 min; t_(1/2β) was 52.3 min; CL It was (3.43±0.22) ml·min~(-1)·kg~(-1); AUC was (593.3±45.7) μg·min·ml~(-1). The pharmacokinetic parameters in the two groups were B (μg There was a significant difference between ml~(-1), t_(1/2)β(min) and V_c(ml·kg~(-1)) (P<0.05). Conclusion: The pharmacokinetics of ferulic acid in Buyang Huanwu Decoction and total aglycone is a two-compartment model. The combination of Chinese herbal compound ingredients has certain influence on pharmacokinetic parameters.