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目的:研究运动性低血色素大鼠小肠铁吸收蛋白二价金属离子转运体1(divalent metal transporter1,DMT1)和膜铁转运蛋白1(ferroportin1,FPN1)表达的变化,探讨运动性低血色素的发生机制。方法:12只雄性Wistar大鼠随机分为对照组(CG)和实验组(EG)。实验组进行5周跑台训练,建立运动性低血色素模型。5周递增负荷跑台运动后,检测两组大鼠血常规和血清铁,采用Western Blot检测小肠上皮细胞DMT1和FPN1表达。结果:(1)实验组Hb显著低于对照组(P<0.01),运动性低血色素造模成功。(2)实验组大鼠小肠上皮细胞DMT1表达比对照组显著降低(P<0.05),FPN1表达比对照组显著下降(P<0.01)。(3)实验组血清铁和转铁蛋白饱合度显著低于对照组(P<0.05,P<0.01)。结论:长时间大强度运动会减少机体肠铁吸收,降低机体运铁能力,是引发运动性低血色素的原因之一。
Objective: To study the changes of expression of divalent metal transporter1 (DMT1) and ferroportin1 (FPN1) in the intestine of rats with exercise - induced hypocholesterolemia and to explore the mechanism of exercise hyphemaemia . Methods: Twelve male Wistar rats were randomly divided into control group (CG) and experimental group (EG). Experimental group for 5 weeks treadmill training, the establishment of exercise hypochromic model. After 5 weeks of incremental load treadmill exercise, blood and serum iron were measured in both groups. The expression of DMT1 and FPN1 in small intestine epithelial cells was detected by Western Blot. Results: (1) The experimental group Hb was significantly lower than the control group (P <0.01), exercise hypochromic model was successful. (2) The expression of DMT1 in experimental group was significantly lower than that in control group (P <0.05), and the expression of FPN1 in experimental group was significantly lower than that in control group (P <0.01). (3) Serum iron and transferrin saturation in the experimental group was significantly lower than that in the control group (P <0.05, P <0.01). Conclusion: Long-term high-intensity exercise will reduce intestinal iron absorption, reduce the body’s ability to transport iron, is one of the reasons trigger exercise hypochromic.