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[目的]希金斯炭疽菌可以侵染十字花科诸多植物引起炭疽病,给各国农业生产造成了巨大经济损失。对该菌候选效应分子进行预测,并对其特征进行明确。[方法]利用Signal P、Prot Comp等程序对该菌中分泌蛋白进行找寻,并对分泌蛋白所具有的氨基酸大小、信号肽长度以及理化性质等进行分析。结合已经报道的真菌效应分子具有的典型特征,明确该菌存在的候选效应分子数量及特征。[结果]希金斯炭疽菌含有658个分泌蛋白,氨基酸长度集中于50~300 aa之间;信号肽长度以17~21个aa的序列最为集中;信号肽切割位点属于A-X-A类型;分泌蛋白在分子量、等电点、不稳定系数等方面均存在差异,但大多数分泌蛋白属于亲水性蛋白。其中,预测功能的蛋白为290个,其功能较多的集中于酶类,包括脂肪酶、α-L-鼠李糖苷酶、果胶酯酶等。依据候选效应分子典型特征,明确该菌中含有388个候选效应分子。[结论]通过上述生物信息学分析方法,结合真菌效应分子典型特征,有效地实现了希金斯炭疽菌候选效应分子的预测,其信号肽切割位点类型与其他已经报道的致病疫霉、粗糙脉孢霉等分泌蛋白信号肽切割位点一致,分泌蛋白功能涉及较多的酶类,也与其他已经报道的病菌分泌蛋白功能相类似。
[Objective] The Higgins anthrax can infect cruciferous plants causing anthracnose and causing great economic losses to agricultural production in all countries. The candidate candidate molecules were predicted, and their characteristics were clarified. [Method] SignalP, Prot Comp and other programs were used to search for secretory proteins in this bacterium. The amino acid size, signal peptide length and physicochemical properties of secretory proteins were also analyzed. Combined with the typical characteristics of fungal effector molecules that have been reported, the numbers and characteristics of candidate effector molecules existing in this strain of bacteria were clarified. [Result] The results showed that Higgins anthrax contained 658 secretory proteins and the length of amino acids was between 50 and 300 aa. The length of the signal peptide ranged from 17 to 21 aa. The signal peptide cleavage site belonged to AXA type. The secretory protein There are differences in molecular weight, isoelectric point, unstable coefficient, etc., but most secreted proteins are hydrophilic proteins. Among them, the predicted function of the protein is 290, its function more concentrated in the enzymes, including lipase, α-L-rhamnosidase, pectin esterase and so on. Based on the typical characteristics of candidate effector molecules, it is clear that the bacteria contain 388 candidate effector molecules. [Conclusion] Through the above bioinformatics analysis methods combined with the typical features of fungal effector molecules, it is effective to predict the candidate effector molecules of Higgins anthrax. The types of signal peptide cleavage sites and other previously reported pathogens such as Phytophthora infestans, Neurospora crassa and other secreted protein signal peptide cleavage site is consistent, the secretion of protein function involves more enzymes, but also with other bacteria have been reported secreted protein function similar.