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目的:通过复制“两次打击”全身炎症反应综合征(SIRS)大鼠模型,探讨迷走神经切断及烟碱预处理对大鼠SIRS的作用,为临床治疗SIRS提供新思路。方法:选择雄性健康SD大鼠40只,随机分为4组,每组10只。A:对照组未予处理;B:单侧迷走神经切断组;C:行右侧颈部迷走神经切断;烟碱预处理组:每天ip烟碱液5 mg·kg-1;连续7 d;D:联合迷走神经切断+烟碱预处理组。7 d后造模(急性放血+Lps ip);造模后12 h取血检测大鼠外周血白细胞计数与分类、肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)、乳酸脱氢酶(LDH)、肌酸磷酸激酶(CPK)、尿素氮(BUN)、肌酐(Cr)、谷丙转氨酶(ALT)、谷草转氨酶(AST)以及胆汁酸(TBA)的变化。结果:迷走神经切断后能提高炎症反应;烟碱预处理后能降低炎症反应;联合二者处理后炎症反应介于两者之间。结论:烟碱在次要的胆碱能抗炎通路中的作用不依赖于迷走神经的完整性,可独立发挥作用。
OBJECTIVE: To investigate the effects of vagotomy and nicotine preconditioning on SIRS in rats by replicating the “two strikes” systemic inflammatory response syndrome (SIRS) rat model to provide new ideas for clinical treatment of SIRS. Methods: Forty healthy male Sprague-Dawley rats were randomly divided into 4 groups with 10 in each group. A: no treatment in the control group; B: unilateral vagotomy group; C: vagotomy in the right side of the neck; nicotine preconditioning group: daily ip-nicotine 5 mg·kg-1 for 7 days; Combined vagotomy + nicotine preconditioning group. After 7 days, the model was established (acute bloodletting + Lps ip); peripheral blood leucocyte counts and classification, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), blood were measured at 12 h after modeling. Changes in lactate dehydrogenase (LDH), creatine phosphokinase (CPK), urea nitrogen (BUN), creatinine (Cr), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and bile acid (TBA). RESULTS: After vagotomy, the inflammatory response was increased, and after nicotine pretreatment, the inflammatory response was reduced. After the combination of the two treatments, the inflammatory response was in between. Conclusion: The role of nicotine in the secondary cholinergic anti-inflammatory pathway does not depend on the integrity of the vagus nerve and may play an independent role.