论文部分内容阅读
目的:探讨VEGF反义寡核苷酸转染对人胆囊癌GBC-SD细胞生长、增殖和凋亡的影响。方法:运用Oligofectamine介导VEGF反义寡核苷酸(antisense oligodeoxynucleotides,ASODN)和错义寡核苷酸(Scrambled Oligodeoxynucleotide,SODN)转染人胆囊癌(GBC-SD)细胞。采用MTT法测定转染后各组细胞的生长曲线及抑制率,流式细胞术检测转染后不同时间各组细胞凋亡情况。结果:SODN组、SODN十Oligofectamine组对GBC-SD细胞生长无显著影响(P>0.05),ASODN组及ASODN十Oligofectamine组则能显著抑制GBC-SD细胞生长(P<0.05),且ASODN十Oligofectamine组对GBC-SD细胞生长增殖抑制作用较ASODN组更为强(P<0.05)。流式细胞术检测结果发现ASODN十Oligofectamine组能促进GBC-SD细胞凋亡(P<0.05)。结论:VEGFASODN转染能抑制胆囊癌GBC-SD细胞生长和增殖,Oligofectamine介导能明显增强VEGF ASODN的抑制作用;Oligofectamine介导VEGF反义寡核苷酸转染能促进胆囊癌GBC-SD细胞的凋亡。
Objective: To investigate the effect of VEGF antisense oligonucleotide transfection on the growth, proliferation and apoptosis of human gallbladder carcinoma GBC-SD cells. Methods: Human gallbladder carcinoma (GBC-SD) cells were transfected with Oligofectamine-mediated VEGF antisense oligodeoxynucleotides (ASODNs) and Scrambled Oligodeoxynucleotides (SODNs). The growth curve and the inhibition rate of each group were determined by MTT assay. The apoptosis of the cells in different groups at different time after transfection was detected by flow cytometry. Results: There was no significant difference in the growth of GBC-SD cells between the SODN group and the SODN-10 Oligofectamine group (P> 0.05). The ASODN group and ASODN-10 Oligofectamine group significantly inhibited the growth of GBC-SD cells The inhibitory effect of GBC-SD cells on proliferation and proliferation of GBC-SD cells was stronger than that of ASODN group (P <0.05). Flow cytometry results showed that ASODN ten Oligofectamine group could promote GBC-SD cell apoptosis (P <0.05). Conclusion: VEGFASODN transfection can inhibit the growth and proliferation of gallbladder GBC-SD cells. Oligofectamine can significantly enhance the inhibitory effect of VEGF ASODN. Oligofectamine-mediated VEGF antisense oligonucleotide transfection can promote the proliferation of GBC-SD cells Apoptosis.