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目的:建立一种测定雷尼替丁、尼扎替丁和西咪替丁3种H2组胺受体药物的高灵敏度的荧光探针新方法。方法:本方法是基于小檗碱和葫芦[7]脲生成稳定的包合物并使其荧光强度显著增强,当加入3种药物中的任何一种均能使葫芦[7]脲/小檗碱包合物的荧光显著猝灭。据此建立了一种以小檗碱为荧光探针测定3种H2组胺受体药物的新方法。结果:药物在一定的浓度范围内与其相应的荧光猝灭值ΔF之间呈良好的线性关系,雷尼替丁、尼扎替丁和西咪替丁检测限分别为0.007,0.007,0.020μg·mL-1,比一般的光谱方法高2个数量级。同时对药物制剂和加标尿样进行测定,获得了满意的回收率。结论:该方法可用于药物制剂和生物体液中上述3种H2组胺受体药物的测定。
OBJECTIVE: To establish a new high-sensitivity fluorescent probe for the determination of three histamine-H2 receptor drugs, ranitidine, nizatidine and cimetidine. Methods: This method is based on berberine and gourd [7] urea to generate a stable inclusion complex and its fluorescence intensity was significantly enhanced when adding any of the three drugs can gourd [7] urea / Berberis The fluorescence of the alkali clathrate is significantly quenched. A new method for the determination of three H2 histamine receptor drugs using berberine as fluorescent probe was established. RESULTS: The drug showed a good linear relationship with the corresponding fluorescence quenching value ΔF at a certain concentration range. The detection limits of ranitidine, nizatidine and cimetidine were 0.007,0.007,0.020μg · mL-1, which is 2 orders of magnitude higher than the average spectroscopic method. At the same time on the pharmaceutical preparations and spiked urine samples were measured, obtained a satisfactory recovery rate. Conclusion: This method can be used for the determination of the above three H 2 histamine receptor drugs in pharmaceutical preparations and biological fluids.