论文部分内容阅读
将纯化的登革３型病毒（ＤＥＮ－３）单克隆抗体３Ｄ３（ＡｂＩ）连结到载体分子ＫＬＨ上，免疫ＢＡＬＢ／ｃ小鼠，取其脾细胞与Ｓｐ２／０小鼠骨髓瘤细胞融合。用ＥＬＩＳＡ夹心法检测阳性克隆，融合率达１００％，阳性率为５％，经２～３次克隆化，获得了６株分泌抗登革病毒单抗的独特型抗体（ＭｃＡｂ２）的杂交瘤细胞系。在进行阳性筛选及特异性鉴定时，选择了以３～５μｇ／ｍｌ的最适Ａｂ１包被浓度，并用正常小鼠Ｉｇ及ＫＬＨ分别包被反应板作对照，以排除其抗异种免疫球蛋白的干扰。
The purified dengue virus type 3 (DEN-3) monoclonal antibody 3D3 (AbI) was linked to the carrier molecule KLH, BALB / c mice were immunized, and the spleen cells were fused with Sp2 / 0 mouse myeloma cells. Positive clones were detected by ELISA sandwich method. The fusion rate was 100% and the positive rate was 5%. After 2 to 3 clones, 6 hybridoma cells secreting anti-dengue virus monoclonal antibody (McAb2) system. In the positive screening and specific identification, the optimal Ab1 coating concentration was selected at 3 ~ 5μg / ml, and the normal mouse Ig and KLH were used to coat the reaction plate as a control to exclude the anti-xenogenic immunoglobulin interference.