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研究 5种艾纳香二氢黄酮类化合物对过氧化损伤的原代培养肝细胞的保护作用。胶原酶灌流法分离大鼠肝细胞。以 CCl4或 Fe SO4+Cys损伤原代培养肝细胞 ,1× 10 - 4 和 1× 10 - 5 mol/ L 的 5种艾纳香二氢黄酮均能明显抑制受损伤细胞的转氨酶逸出、MDA产生及 GSH耗竭。其中以 2 ,5 -二羟基艾纳香二氢黄酮的作用最强 ,在 CCl4所致肝细胞损伤实验中 ,其抑制 MDA产生的 ED5 0 约为 2 .6× 10 - 6 mol/ L ,保护 GSH的 ED5 0 约为 1.0 8× 10 - 5 mol/ L ,抑制 AL T逸出细胞的 ED5 0 约为 9.39× 10 - 6 mol/ L
To study the protective effect of five kinds of flavonoids on the primary cultured hepatocytes of peroxidation injury. Collagenase perfusion was used to isolate rat hepatocytes. Primary cultured hepatocytes were injured by CCl4 or Fe SO4+Cys. Five kinds of flavonoids, 1×10 -4 and 1× 10 -5 mol/L, both significantly inhibited transaminase escape from damaged cells. Production and GSH depletion. Among them, 2,5-dihydroxy-indanflavanone had the strongest effect. In the experiment of hepatocyte injury induced by CCl4, the ED50 that inhibits MDA production was about 2. 6×10 - 6 mol/L. The ED50 of GSH is approximately 1.08×10 -5 mol/L, and the ED50 that inhibits AL T escaped cells is approximately 9.39×10 -6 mol/L.