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目的探讨成年大鼠海马CA1区损毁后,齿状回(DG)细胞先增殖后减少的原因。方法用海人酸(KA)建立单侧海马损毁模型;用5-溴脱氧尿嘧啶核苷(BrdU)标记海马DG区增殖的细胞,用免疫组织化学方法检测标记后不同时间点DG区BrdU阳性细胞,并用无偏差体视学方法定量分析;激光共聚焦显微镜检测BrdU与裂解的caspase-3的共表达。结果实验组动物在损毁海马后各时间点DG区BrdU阳性细胞总数均比对照组明显增多,差异有统计学意义(P<0.05);各组动物BrdU阳性细胞总数在标记后第1天最多,随着时间的延长逐渐减少;在颗粒细胞层和门区均有少量BrdU/cleaved caspase-3共表达的阳性细胞。结论损毁成年大鼠海马CA1区后,增殖的细胞总数随着时间的延长逐渐减少,其机制可能与caspase-3相关的细胞死亡及异位迁移有关。
Objective To investigate the reason why the dentate gyrus (DG) cells proliferate first and then decrease in hippocampal CA1 area of adult rats. Methods Unilateral hippocampal damage model was established by kainate (KA). BrdU-labeled cells were used to detect the proliferation of hippocampal DG cells. Immunohistochemistry was used to detect BrdU positive cells in DG area Cells were quantified by unbiased stereological methods. The co-expression of BrdU and cleaved caspase-3 was detected by laser confocal microscopy. Results The total numbers of BrdU positive cells in DG area of experimental group were significantly higher than that of control group at each time point (P <0.05). The total number of BrdU positive cells in each group was the highest on the first day after labeling, With the extension of time gradually decreased; a small amount of BrdU / cleaved caspase-3 co-expression of positive cells in the granular cell layer and the gate area. Conclusion The total number of proliferating cells in the hippocampal CA1 region of adult rats is gradually decreased after prolonged. The mechanism may be related to cell death and ectopic migration related to caspase-3.