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目的对嗜水气单胞菌气溶素基因进行检测并分析其携带率。方法采用常规法从三角帆蚌体内分离嗜水气单胞菌并鉴定,PCR法扩增气溶素毒力基因,计算其携带率。结果5株嗜水气单胞菌携带aer毒力基因,携带率为50%。结论气溶素基因是嗜水气单胞菌的重要毒力因子。
Objective To detect the aerosol gene of Aeromonas hydrophila and analyze its carrier rate. Methods Aeromonas hydrophila was isolated from Hyriopsis cumingii by conventional methods and its virulence genes were amplified by PCR. The carrier rate was calculated. Results Five strains of Aeromonas hydrophila carried the vir virulence gene, carrying rate of 50%. Conclusion The aerosol gene is an important virulence factor of Aeromonas hydrophila.