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目的 :用荧光定量聚合酶链反应 (FQ PCR)准确地定量检测孕妇血清中人巨细胞病毒 (HCMV)的数量及其在临床上的意义。方法 :用FQ PCR和ELISA方法同时检测 30 0例孕妇及 5 9例新生儿血清标本。结果 :胎儿宫内感染的发生与孕妇血清中HCMVDNA数量有关 ,发生宫内胎儿感染的孕妇 ,其HCMVDNA拷贝数达 10 6copies/ml以上。结论 :孕妇血清中HCMVDNA含量升高是胎儿发生HCMV感染的重要因素之一。FQ PCR可以准确定量检测HCMV的真实感染和复制情况 ,对于临床诊断与治疗有一定的指导意义。
Objective: To quantitatively detect the quantity of human cytomegalovirus (HCMV) in pregnant women by fluorescence quantitative polymerase chain reaction (FQ PCR) and its clinical significance. Methods: Serum samples from 30 0 pregnant women and 59 newborns were detected simultaneously by FQ PCR and ELISA. Results: The incidence of intrauterine infection was related to the amount of HCMVDNA in serum of pregnant women. The pregnant women with intrauterine fetal infection had copies of HCMVDNA above 106copies / ml. Conclusion: The increase of serum HCMVDNA in pregnant women is one of the important factors of HCMV infection in fetus. FQ PCR can accurately detect the true infection and replication of HCMV, which has some guiding significance for clinical diagnosis and treatment.