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目的:探讨大黄酸和吡格列酮各自对高脂诱建的大鼠非酒精性脂肪性肝炎(NASH)的影响及其可能的机制.方法:52只清洁级雄性SD大鼠,体重140～160 g,随机分成4组:对照组(n=14)、模型组(n=14)、大黄酸组(n=12)、吡格列酮组(n=12).模型组由高脂高胆固醇饲料(普通饲料+10%猪油+2%胆固醇配制)喂养,药物组均在12周高脂饮食后即造模成功后给予干预.大黄酸用生理盐水配成5 g/L混悬液,每天固定时间 100 mg·kg~(-1)·d~(-1)灌胃,吡格列酮组8 mK·kg~(-1)·d~(-1)灌胃.于第20周处死.测定所有动物体重、肝湿重、计算肝指数;测定空腹血糖(FBG)、转氨酶及血脂水平;放射免疫法测空腹胰岛素(FINS)及TNF-α,计算胰岛素抵抗指数;酶法测定肝组织匀浆丙二醛(MDA)、谷胱甘肽过氧物酶(GSH-Px)水平;HE染色肝病理组织切片.结果:与模型组相比,药物组ALT、AST、血糖、胰岛素及胰岛素抵抗指数、TNF-α、MDA有明显下降;大黄酸组肝脂肪变较吡格列酮组明显(P＜0.05),但其肝内炎症较吡格列酮组轻.结论:大黄酸和吡格列酮对大鼠NASH均有治疗作用,但在改善肝脂肪变及炎症方面有所不同.“,”AIM: To approach the therapeutic effect of rhein and pioglitazone respectively on nonalcoholic steatosis hepatitis (NASH) rats and the possible mechanism. METHODS: 52 male SD rats were randomly divided into 4 groups: control group (n = 14), model group (n = 14), rhein group (n = 12), pioglitazone group (n = 12), the body weight were 140 - 160 g. Control group were fed with normal diet for 20 weeks (2 rats for 12 weeks and then were executed) . Model group were fed with high-fat and high-cholesterol diet consisting of normal diet, 10% lard and 2% cholesterol, for 20 weeks (2 rats for 12 weeks and then were executed) . Drugs were given after 12 weeks fed with high-fat and high-cholesterol diet. Rhein (5 g/L suspension) was prepared with normal saline, intragas-tric administration (100 mg·kg~(-1)·d~(-1)) everyday in fixation time. Pioglitazone group (8 mg·kg~(-1)·d~(-1)) were intragastric administered. All rats were executed after 20 weeks, and the body weight, liver humid weight were determined, the liver index number was calculated . The levels of fasting blood glucose (FBG), amin-opherase and blood fat were determined. The fasting insulin (FINS) and TNF-a were measured by radio-im-munoassay, the insulin resistance index was calculated . The levels of malonaldehyde (MDA), glutathione peroxidase(GSH-Px)in hepatic tissue bomogenate were measured using enzymic method, the liver histopatho-logic slides were dyed with HE dyeing. RESULTS: Compared with model group, the levels of ALT, AST, blood glucose, insuline, insulin resistance index, TNF-ct, MDA were obviously decreased in drug groups. Compared with pioglitazone group, the hepatic steatosis in rhein was obvious(P < 0.05), but the inflammation in liver was lower grade. CONCLUSION: Rehin and pioglitazone have therapeutical effects on NASH rats. However, they are different in the aspects of improving hepatocyte steatosis and inflammatory.