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目的探讨体外三磷酸腺苷(ATP)、神经生长因子(NGF)对缺氧复氧大鼠皮质神经元的保护作用。方法取体外培养8d的Wistar大鼠皮质神经元,随机分为正常对照组、缺氧组、ATP组和NGF组。用噻唑盐比色法(MTT)、乳酸脱氢酶(LDH)法测定细胞活性变化,流式细胞仪术、透射电镜检测细胞凋亡率和凋亡。结果与对照组比较,缺氧组神经元细胞活性明显降低,细胞凋亡率显著增加;ATP和NGF能明显改善缺氧对神经元细胞活性的影响,细胞凋亡率降低(P<0.05);与NGF组比较,ATP组神经元细胞活性增高,细胞凋亡率降低(P<0.05)。结论缺氧能够造成大鼠大脑皮质神经元的损伤并诱导其凋亡,ATP及NGF对缺氧复氧后大脑皮质神经元有保护作用。
Objective To investigate the protective effect of adenosine triphosphate (ATP) and nerve growth factor (NGF) on cortical neurons in anoxia-reoxygenation rats. Methods Cortical neurons of Wistar rats cultured in vitro for 8 days were randomly divided into normal control group, hypoxia group, ATP group and NGF group. The cell viability was measured by MTT assay and LDH assay. The apoptosis rate and apoptotic rate were detected by flow cytometry and transmission electron microscopy. Results Compared with the control group, the activity of neurons in hypoxia group was significantly decreased and the apoptosis rate was significantly increased. The effect of hypoxia on neuronal cell viability was significantly attenuated by ATP and NGF (P <0.05). Compared with NGF group, the activity of neurons in ATP group increased and the apoptosis rate decreased (P <0.05). CONCLUSION: Hypoxia can cause neuronal damage and induce apoptosis in rat cerebral cortex. ATP and NGF may protect cerebral cortex neurons after hypoxia-reoxygenation.