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目的研究抗癌药物黄药子Dioscorea bulbifera的茋类化学成分。方法采用硅胶柱色谱、ODS、Sephadex LH-20柱色谱以及HPLC制备方法进行分离纯化,通过理化性质及波谱数据分析鉴定了化合物的结构。结果从黄药子95%乙醇提取物的醋酸乙酯萃取部位中分离得到14个茋类化合物,分别鉴定为2,7-二羟基-3,4-二甲氧基-9,10-二氢菲(1)、2,5-二羟基-4-甲氧基-9,10-二氢菲(2)、latifolin(3)、2′,3-二羟基-4,5-二甲氧基联苄(4)、4-甲氧基菲-2,3,7-三醇(5)、3,7-二羟基-2,4-二甲氧基菲(6)、2′,3-二羟基-5-甲氧基联苄(7)、3,4′,5-三羟基-3′-甲氧基联苄(8)、7-羟基-2,3,4-三甲氧基菲(9)、3,3′-二羟基-5-甲氧基联苄(10)、2,7-二羟基-4-甲氧基-9,10-二氢菲(11)、2,7-二羟基-3,4-二甲氧基菲(12)、2,7-二羟基-2,4-二甲氧基-9,10-二氢菲(13)、4,7-二羟基-2-甲氧基-9,10-二氢菲(14)。结论化合物1~3、5~7、9、12、14均为首次从该植物中分离得到。
Objective To study the chemical constituents of the anti-cancer drug Dioscorea bulbifera. Methods The compounds were isolated by silica gel column chromatography, ODS, Sephadex LH-20 column chromatography and HPLC. The structures of the compounds were identified by physicochemical properties and spectral data analysis. Results Fourty compounds were isolated from the ethyl acetate extract of 95% ethanol extract of Xanthium sibiricum and identified as 2,7-dihydroxy-3,4-dimethoxy-9,10-dihydrophenanthrene 1), 2,5-dihydroxy-4-methoxy-9,10-dihydrophenanthrene (2), latifolin (3), 2 ’, 3-dihydroxy-4,5-dimethoxybibenzyl Methoxyphenanthrene-2,3,7-triol (5), 3,7-dihydroxy-2,4-dimethoxyphenanthrene (6), 2 ’, 3-dihydroxy Methoxybibenzyl (7), 3,4 ’, 5-trihydroxy-3’-methoxybibenzyl (8), 7-hydroxy- Dihydroxy-5-methoxybenzyl (10), 2,7-dihydroxy-4-methoxy-9,10-dihydrophenanthrene (11), 2,7-bis (12), 2,7-dihydroxy-2,4-dimethoxy-9,10-dihydrophenanthrene (13), 4,7-dihydroxy-2 -methoxy-9,10-dihydrophenanthrene (14). Conclusion Compounds 1 ~ 3,5 ~ 7,9,12,14 were isolated from this plant for the first time.