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目的:探讨清热凉血养阴祛风法对佐剂性关节炎大鼠外周血T淋巴细胞亚群及相关细胞因子TNF-α、IL-1β的影响。方法:取SD大鼠制成佐剂性关节炎模型,设正常组、模型组、痹颗粒组、实验组。造模后第7天起分别予痹颗粒1.88 g/(kg·d),实验方29 g/(kg·d),正常组、模型组予生理盐水3 mL/(只·d),连续灌胃3周。腹主动脉取血,用流式细胞技术检测外周血中T淋巴细胞亚群(CD3+CD4+、CD3+CD8+、CD4+/CD8+),用酶联免疫吸附法(ELISA)检测外周血血清中细胞因子TNF-α及IL-1β。结果:实验组CD4+、CD8+T淋巴细胞,CD4+/CD8+的比值与模型组比较差异有显著性(P<0.01或P<0.05),CD4+淋巴细胞水平降低,CD8+淋巴细胞水平升高,CD4+/CD8+的比值降低。实验组细胞因子TNF-α及IL-1β水平与模型组比较差异有显著性(P<0.01),TNF-α、IL-1β水平降低。结论:清热凉血、养阴祛风法能调节T淋巴细胞紊乱和相关细胞因子水平,对大鼠佐剂性关节炎有治疗作用。
Objective: To investigate the effect of clearing away heat, cooling blood, nourishing Yin and expelling wind on peripheral T lymphocyte subsets and related cytokines TNF-α and IL-1β in adjuvant arthritis rats. Methods: SD rats were made adjuvant arthritis model, set the normal group, model group, Bi particles group, the experimental group. On the 7th day after model establishment, the rats were given pellets of 1.88 g / (kg · d) and experimental group (29 g / (kg · d) respectively). Normal group was given normal saline (3 mL / Stomach for 3 weeks. Blood samples were collected from the abdominal aorta, T lymphocyte subsets (CD3 + CD4 +, CD3 + CD8 +, CD4 + / CD8 +) in peripheral blood were detected by flow cytometry. Serum levels of cytokines were detected by enzyme-linked immunosorbent assay (ELISA) TNF-α and IL-1β. Results: The ratio of CD4 +, CD8 + T lymphocytes and CD4 + / CD8 + in the experimental group was significantly lower than that in the model group (P <0.01 or P <0.05), the level of CD4 + lymphocytes was decreased, the level of CD8 + CD8 + ratio decreased. The levels of TNF-α and IL-1β in the experimental group were significantly lower than those in the untreated group (P <0.01), while the levels of TNF-α and IL-1β were decreased. Conclusion: The method of clearing away heat and cooling blood and nourishing Yin and expelling wind can regulate the disturbance of T lymphocyte and related cytokines and has therapeutic effect on adjuvant arthritis in rats.