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目的探讨人乳头状瘤病毒(HPV)16/18、单纯疱疹病毒Ⅱ型(HSVⅡ)和巨细胞病毒(CMV)感染与宫颈癌发生、发展的关系。方法对43例宫颈癌、47例宫颈上皮瘤样病变(CIN)、56例宫颈炎和10例正常宫颈进行聚合酶链反应检测HPV16/18、HSVⅡ和CMV感染情况。结果HSVⅡ、HPV16/18和CMV的阳性率在宫颈癌和CIN组、CINⅢ级和CINⅠ～Ⅱ级组均有递减趋势,且差异有统计学意义。Ⅱ期宫颈癌组HPV16/18阳性显著高于Ⅰ期宫颈癌组,高分化宫颈癌组HPV16/18和HSVⅡ阳性高于中分化组,与临床分期及组织类型差异均无统计学意义;CMV阳性与临床分期、组织分级及组织类型差异均无统计学意义。三种病毒感染拷贝数,HSVⅡ和HPV16/18:宫颈癌>CIN>宫颈炎;CMV:宫颈癌>CIN。宫颈癌中出现几种病毒混合感染,其中HPV16/18合并HSVⅡ明显多于HPV16/18合并CMV者。结论HPV16/18、HSVⅡ和CMV感染与宫颈癌的发生发展关系密切,且与病毒负荷量有关,可能是宫颈癌的致病因子。
Objective To investigate the relationship between human papillomavirus (HPV) 16/18, herpes simplex virus type 2 (HSV Ⅱ) and cytomegalovirus (CMV) infection and the occurrence and development of cervical cancer. Methods 43 cases of cervical cancer, 47 cases of cervical epithelial neoplasia (CIN), 56 cases of cervicitis and 10 cases of normal cervix were tested for HPV16 / 18, HSV Ⅱ and CMV infection by polymerase chain reaction. Results The positive rate of HSVⅡ, HPV16 / 18 and CMV in cervical cancer and CIN group, CIN Ⅲ and CIN Ⅰ ~ Ⅱ group showed a decreasing trend, and the difference was statistically significant. The positive rate of HPV16 / 18 in stage Ⅱ cervical cancer was significantly higher than that in stage Ⅰ cervical cancer, and the positive rate of HPV16 / 18 and HSVⅡ in well-differentiated cervical cancer was higher than that in moderately differentiated group, but not significantly different from the clinical stage and the type of tissue. There was no significant difference with clinical stage, histological grade and type of tissue. Three copies of virus infection, HSV Ⅱ and HPV16 / 18: cervical cancer> CIN> cervicitis; CMV: cervical cancer> CIN. There are several mixed virus infections in cervical cancer, including HPV16 / 18 combined with HSV Ⅱ significantly more than those with HPV16 / 18 CMV. Conclusion HPV16 / 18, HSV Ⅱ and CMV infection are closely related to the occurrence and development of cervical cancer, and are related to the viral load, which may be the causative agent of cervical cancer.