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目的:研究院内艰难梭菌毒素分离株的毒素情况,对核糖体分型展开研究。方法:抽选出100例腹泻病住院患者未成形的大便制成标本后,完成艰难梭菌的分离培养,鉴定菌落;提取出菌株DNA,并采用PCR法扩增毒素基因tcdA和tcdB;并对核糖体进行分型研究。结果:100例患者培养阳性率为15.00%,共分离出55株艰难梭菌,产毒株占比83.64%;毒素类型集中为A+B+型,占比54.55%;核糖体分型检测相对优势为R4型占比18.18%;R8型占比21.82%。结论:A+和B+毒素型为主要艰难梭菌型;住院腹泻患者主要为院内感染,核糖体分型未提示暴发流行。
Objective: To study the toxin status of the C. difficile toxin isolates in the hospital and to study the ribosome typing. Methods: 100 cases of diarrhea inpatients with unformed stool samples were selected to complete the isolation and culture of Clostridium difficile, and the colonies were identified. The DNA of the strains was extracted and the toxins tcdA and tcdB were amplified by PCR Ribosomal typing studies. Results: The positive rate of culture in 100 patients was 15.00%, 55 strains of C. difficile were isolated, accounting for 83.64% of strains producing toxins; the type of toxins was A + B + type, accounting for 54.55%; the relative advantage of ribotype typing R4 type accounted for 18.18%; R8 type accounted for 21.82%. CONCLUSIONS: The A + and B + toxin types are predominantly C. difficile; inpatient diarrhea is predominantly nosocomial and ribotyping does not suggest an outbreak.