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应用细胞培养、免疫组化、逆转录多聚酶链式反应 (ＲＴ -ＰＣＲ)技术 ,观察缺氧和 /或谷氨酸对体外培养的星形胶质细胞表达细胞色素Ｐ450 2ｃ1 1ｍＲＮＡ的影响 ,探讨大脑星形胶质细胞在缺氧性脑血管扩张反应中的作用。实验用新生 2 - 3ｄＷｉｓｔａｒ大鼠 ,取双侧大脑皮层组织
The effects of hypoxia and / or glutamate on the expression of cytochrome P450 2c1 1 mRNA in cultured astrocytes were observed by cell culture, immunohistochemistry and reverse transcriptase-polymerase chain reaction (RT-PCR) Role of astrocytes in hypoxic cerebrovascular dilatation. Experimental neonatal 2 - 3dWistar rats, bilateral cerebral cortex tissue