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目的:建立快速准确的头花蓼与尼泊尔蓼药材的分子标记鉴定方法。方法:通过RAPD随机引物筛选,获得头花蓼特异的RAPD分子标记片段,经克隆、测序,重新设计特异性引物转化形成稳定的SCAR标记。结果:获得2条稳定扩增的头花蓼特异片段Z300,Z1100,根据该2特异片段序列设计4对特异引物,分别对头花蓼与尼泊尔蓼药材DNA进行扩增,其中引物Z1-2可从头花蓼药材中扩增获得约300 bp的片段,而尼泊尔蓼药材则未扩增出该片段。结论:引物Z1-2成功将Z300片段转化为SCAR分子标记,可作为头花蓼与近缘种尼泊尔蓼药材分子鉴定的依据。
OBJECTIVE: To establish a rapid and accurate method for molecular marker identification of Polygonum capitatum and Polygonum sibiricum in Nepal. Methods: RAPD-specific RAPD molecular marker fragments were obtained by RAPD random primer screening. After cloning, sequencing and redesigning specific primers, a stable SCAR marker was obtained. Results: Two amplified fragments of Z300 and Z1100 were obtained. Two pairs of specific primers were designed according to the sequence of two specific fragments. The primers were used to amplify the DNA of Polygonum capitatum and Polygonum sibiricum respectively. Among them, primer Z1-2 could be de novo Polygonum herbs amplified about 300 bp fragments, while the Polygonum sibiricum did not amplify the fragment. Conclusion: Primer Z1-2 successfully transformed Z300 fragment into SCAR molecular marker, which can be used as the basis for molecular identification of Polygonum capitatum and its related species.