通过分子设计和过程策划,制备了高性能的牛血清白蛋白(BSA)分子表面印迹材料.首先以甲基丙烯酰氯为试剂,使交联聚乙烯醇(CPVA)微球表面的羟基发生酯化反应,将大量可聚合双键引入到CPVA微球表面.然后以含有可聚合双键的CPVA微球为载体,阳离子单体丙烯酰氧乙基三甲基氯化铵(DAC)为功能单体,N,N’-亚甲基双丙烯酰胺(MBA)为交联剂,牛血清白蛋白(BSA)为模板分子,在水溶液体系中,基于主-客体之间的强静电相互作用,采用接枝聚合与印迹过程同步进行的方式,制备了高性能BSA分子表面印迹微球MIP-PDAC/CPVA.采用红外光谱(IR)和扫描电子显微镜(SEM)对产物微球进行了表征.研究了印迹聚合物微球MIP-PDAC/CPVA对BSA的大分子识别特性.研究结果表明,微球MIP-PDAC/CPVA对BSA具有优良的结合亲和性和特异的识别选择性,结合容量高达108 mg/g,对牛血红蛋白(BHb)却基本不结合;相对于BHb,MIP-PDAC/CPVA对BSA的选择性系数高达60.2. High performance bovine serum albumin (BSA) molecular imprinting material was prepared by molecular design and process planning.Firstly, hydroxyl groups on the surface of crosslinked polyvinyl alcohol (CPVA) microspheres were esterified with methacrylic chloride Reaction, a large number of polymerizable double bond introduced into the surface of the CPVA microspheres.Then the CPVA microspheres containing polymerizable double bonds as the carrier, the cationic monomer acryloyloxyethyl trimethyl ammonium chloride (DAC) as a functional monomer , N, N’-methylenebisacrylamide (MBA) as crosslinking agent and bovine serum albumin (BSA) as template molecule. In aqueous solution system, based on the strong electrostatic interaction between host and guest, The microspheres MIP-PDAC / CPVA with high molecular weight were prepared by the simultaneous polymerization of the dendrimer and the imprinting process.The product microspheres were characterized by infrared spectroscopy (IR) and scanning electron microscopy (SEM) MIP-PDAC / CPVA polymer macromolecules recognition properties of BSA.The results show that microspheres MIP-PDAC / CPVA BSA has excellent binding affinity and specificity of the recognition selectivity of binding capacity of up to 108 mg / g, but not to bovine hemoglobin (BHb); compared with BHb, MIP-PDAC / The selectivity coefficient of CPVA to BSA is up to 60.2.