论文部分内容阅读
目的：研究维生素Ｃ、谷胱甘肽、秦美种猕猴桃汁３种抗氧化剂对铬（Ｃｒ）所致细胞毒性、ＤＮＡ损伤及Ｃｒ价态的影响。方法：应用噻唑蓝比色法、单细胞凝胶电泳及原子吸收法研究Ｃｒ的细胞毒性、ＤＮＡ损伤与价态的变化及３种抗氧化剂的干预作用。结果：猕猴桃汁可以有效地拮抗Ｃｒ６＋引起的细胞毒性，促进Ｃｒ６＋在细胞外的还原，降低Ｃｒ６＋的细胞摄入，而维生素Ｃ及谷胱甘肽的作用则随Ｃｒ染毒剂量的不同而不同。结论：猕猴桃汁对Ｃｒ所致细胞生物学效应的拮抗作用明显优于维生素Ｃ及谷胱甘肽，可能与其促进Ｃｒ６＋细胞外还原，阻断Ｃｒ６＋细胞摄入有关。
Objective: To study the effects of three antioxidants such as Vitamin C, Glutathione and Qin Kiwi juice on the cytotoxicity, DNA damage and Cr valence caused by Cr. Methods: Cytotoxicity, DNA damage and valence of Cr were studied by MTT assay, single cell gel electrophoresis and atomic absorption spectrophotometry. The effects of three antioxidants were also studied. Results: Kiwi juice can effectively antagonize the cytotoxicity induced by Cr6 +, promote the extracellular reduction of Cr6 +, and reduce the cellular uptake of Cr6 +. However, the effect of vitamin C and glutathione varies with the dose of Cr. CONCLUSION: Kiwifruit juice has a significantly better antagonistic effect on cytotoxicity induced by Cr than that of vitamin C and glutathione, which may be related to the promotion of extracellular reduction of Cr6 + and the uptake of Cr6 + cells.