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以兔IgG为模型 ,NaTPB与PPP协同增强的Luminol H2 O2 HRP混合液为发光体系 ,HRP标记抗体 ,建立了灵敏度高、特异性强、重现性好的流动注射协同增强化学发光免疫分析法。结果表明 :在 2～ 6 0 μg L范围内兔IgG量与发光强度有良好的线性关系 ,相关系数r=0 .994 1 (P <0 .0 1 ) ;绝对检测限为 0 .6 5fmol;方法精密度为 4 .72 %～ 9.31 % ;回收率为 92 .5 0 %～ 99.4 0 %。免疫柱可反复使用 2 0 0次以上。本法测定不同浓度兔IgG标准溶液的结果与RIA法的测定结果基本一致
Rabbit IgG as a model, NaTPB and PPP synergistic LuminolH2 O2 HRP mixture of luminescence system, HRP-labeled antibody, the establishment of a high sensitivity, specificity and reproducibility of flow injection synergistic chemiluminescence immunoassay. The results showed that there was a good linear relationship between the amount of IgG and the luminescence intensity in the range of 2 ~ 60 μg L, the correlation coefficient r = 0.994 1 (P <0.01); the absolute detection limit was 0.6 6 fmol; The precision of the method was from 4.72% to 9.31%, and the recovery was from 92.5% to 99.4%. Immunization column can be used repeatedly more than 200 times. The results of determination of different concentrations of rabbit IgG standard solution with the determination of the results of RIA method is basically the same