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目的分析特发性血小板减少症(ITP)患儿抗核抗体(ANA)及可提取性核抗原(ENA)多肽抗体谱特点。方法选取2013年1月—2014年10月在广州市妇女儿童医疗中心血液及免疫科门诊就诊或住院的初发ITP患儿67例为ITP组,另选取同期在该院健康体检的50例健康儿童为对照组,分别应用间接免疫荧光法和蛋白印迹法检测ANA及ENA多肽抗体谱。结果 ITP组患儿ANA阳性率、抗ENA抗体阳性率均高于对照组(P<0.01);ANA阳性ITP患儿治疗前、后血小板计数及增值均低于ANA阴性患儿,且ANA阴性ITP患儿临床疗效优于ANA阳性患儿(P<0.05)。结论检测ANA可反映ITP患儿对治疗的反应,ANA、抗SSA抗体等可能与ITP的发病及病情进展有关。
Objective To analyze the characteristics of antibody spectrum of antinuclear antibody (ANA) and extractable nuclear antigen (ENA) in children with idiopathic thrombocytopenia (ITP). Methods From January 2013 to October 2014, 67 children with ITP who were treated or hospitalized in the Department of Hematology and Immunology of Guangzhou Women and Children’s Medical Center were selected as ITP group. Another 50 healthy people Children as control group, indirect immunofluorescence and Western blotting were used to detect antibody spectrum of ANA and ENA respectively. Results The positive rate of ANA and the positive rate of anti-ENA antibody in children with ITP were significantly higher than those in control (P <0.01). The counts and the increment of platelet counts in patients with ANA-positive ITP before and after treatment were lower than those in children with ANA-negative and ANA-negative ITP The clinical efficacy of children with ANA-positive children (P <0.05). Conclusion The detection of ANA can reflect the response of ITP children to treatment, ANA, anti-SSA antibodies may be related to the incidence of ITP and progression of the disease.