论文部分内容阅读
目的以人血清白蛋白为载体制备藤黄酸白蛋白纳米粒,对处方和工艺进行优化,并进行稳定性考察。方法采用新型白蛋白纳米制备技术(Nab~(TM))制备藤黄酸白蛋白纳米粒(GA-HSA NPs),用透射电镜观察纳米粒的形态,用Nicomp TM PSS 380进行粒度仪测定其粒度分布及电位,超速离心法测定其包封率、载药率及过膜效率,并考察其稀释稳定性及储存稳定性。结果 GA-HSA NPs电镜下呈球状粒子,粒径为(103.2±34.4)nm,zeta电位为-25.10 mV,GA-HSA NPs具有较高的包封率、载药率和过膜效率。GA-HSA NPs冻干粉针储存12个月期间无塌陷或皱缩,GA-HSA NPs混悬液在储存6周内粒径无显著性变化。结论难溶性抗癌药物藤黄酸可以采用NabTM制备成白蛋白纳米粒,其粒径小,稳定性高,有望成为藤黄酸的新型给药系统。
OBJECTIVE To prepare human albumin as carrier, and to optimize the prescription and process, and to study the stability. Methods Gambogic acid albumin nanoparticles (GA-HSA NPs) were prepared by a novel albumin nanoparticle preparation technology (Nab ~ (TM)). The morphology of the nanoparticles was observed by transmission electron microscopy and the particle size Distribution and potential, ultracentrifugation assay encapsulation efficiency, drug loading rate and membrane efficiency, and to investigate the dilution stability and storage stability. Results GA-HSA NPs were spherical under electron microscope. The particle size was (103.2 ± 34.4) nm and the zeta potential was -25.10 mV. GA-HSA NPs had higher entrapment efficiency, drug loading rate and membrane efficiency. The GA-HSA NPs freeze-dried powder stored for 12 months without collapse or collapse, GA-HSA NPs suspension particle size within 6 weeks of storage no significant change. Conclusions Galenic acid, a poorly-soluble anticancer drug, can be prepared into albumin nanoparticles by using NabTM. Its particle size is small and its stability is high. It is expected to be a new drug delivery system for gambogic acid.