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一、Vero 细胞分为三组,第一组细胞营养阶段含硒(1微克/毫升),维持液不含硒;第二组细胞营养阶段及维持阶段均含硒 (0.1微克/毫升);第三组细胞营养阶段不含硒,维持液含硒(0.1微克/毫升),细胞对柯萨奇病毒的敏感性没有改变。二、每毫升含有0.05微克亚砸酸钠的营养液用作连续传代 Vero 细胞,细胞可以正常传代下去,这样的细胞株对病毒的敏感性有一定程度降低。这种敏感性降低的原因不是硒对病毒直接作用,而是细胞本身敏感性发生改变。
One, Vero cells were divided into three groups, the first group of cell nutrition stage containing selenium (1 microgram / ml), the maintenance solution does not contain selenium; the second group of cells nutrition and maintenance phase selenium (0.1 micrograms / ml) Three groups of cell nutrition stage does not contain selenium, selenium maintenance solution (0.1 micrograms / ml), the cell sensitivity to Coxsackie virus has not changed. Second, each milliliter contains 0.05 microliters of sodium metabisulfite nutrient solution for continuous passage Vero cells, the cells can be passed on, the sensitivity of this cell line to the virus to a certain extent. The reason for this decrease in sensitivity is not that selenium acts directly on the virus, but rather that the cell’s own sensitivity changes.