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目的比较人透明带糖蛋白、孕酮和钙离子载体A23187在体外诱导人精子顶体反应的效率和可行性。方法通过精子上游法制备正常男性精子悬液,分别以人透明带糖蛋白、孕酮和钙离子载体A231 87诱导精子发生顶体反应。随后,运用考马斯亮蓝染色判断精子顶体的状态,并统计精子的顶体反应率。结果人透明带糖蛋白、孕酮和钙离子载体A231 87三法诱导的人精子顶体反应率分别为:(32.45±6.92)%(n=11)、(42.4 1±1 9.1 9)%(n=22)和(6 8.24±8.8 5)%(n=43),经统计学分析各组间差异均有统计学意义(P<0.05)。结论三种方法中,钙离子载体A231 87诱导效果最好,且不同个体间差异较小;而孕酮诱导的精子顶体反应率在不同个体间差异较大。
Objective To compare the efficiency and feasibility of using human zona pellucida, progesterone and calcium ionophore A23187 to induce human sperm acrosome reaction in vitro. Methods The normal male sperm suspension was prepared by sperm upstream method, and the sperm acrosome reaction was induced by human zona pellucida glycoprotein, progesterone and calcium ionophore A231 87 respectively. Subsequently, the use of Coomassie brilliant blue staining to determine sperm acrosome status, and sperm acrosome reaction rate statistics. Results The acrosome reaction rates of human zona pellucida induced by human zona pellucida glycoprotein, progesterone and calcium ionophore A231 87 were (32.45 ± 6.92)% (n = 11), (42.4 ± 1 9.19)% n = 22) and (6 8.24 ± 8.85)% (n = 43) respectively. There was significant difference among the groups by statistical analysis (P <0.05). Conclusions Among the three methods, calcium ionophore A23187 has the best induction effect and the difference between different individuals is small. Progesterone-induced sperm acrosome reaction rate varies greatly between different individuals.