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目的评价尿脱落细胞中LivinαmRNA表达对诊断膀胱尿路上皮癌的价值。方法选择43例膀胱尿路上皮癌患者(病例组)和31例非尿路上皮肿瘤患者(对照组),分别进行尿脱落细胞的LivinαmRNA检测(逆转录多聚酶链反应法)和尿细胞学检查。分析尿脱落细胞中LivinαmRNA的表达及其与肿瘤病理分级和分期的关系,比较两种诊断性试验的敏感度、特异度和Youden指数。结果病例组尿脱落细胞中LivinαmRNA的阳性表达率为81.4%(35/43),明显高于对照组的6.5%(2/31)(P<0.05);病例组尿脱落细胞中LivinαmRNA表达与肿瘤的病理分级和分期无关(P>0.05)。尿脱落细胞LivinαmRNA检测的敏感度和Youden指数均显著高于尿细胞学检查(P<0.05),而两者的特异度差异无统计学意义(P>0.05)。结论检测尿脱落细胞LivinαmRNA可作为膀胱尿路上皮癌较理想的无创性诊断方法。
Objective To evaluate the value of LivinαmRNA expression in exfoliated urothelial cells in the diagnosis of bladder urothelial carcinoma. Methods 43 patients with bladder urothelial carcinoma (case group) and 31 patients with non-urothelial tumors (control group) were selected for detection of LivinαmRNA in urine exfoliated cells (RT-PCR) and urine cytology. The expression of LivinαmRNA in urine exfoliated cells and its relationship with tumor grade and staging were analyzed. The sensitivity, specificity and Youden index of the two diagnostic tests were compared. Results The positive rate of LivinαmRNA in exfoliated cells was 81.4% (35/43), which was significantly higher than that in control group (6.5%, 2/31) (P <0.05). The expression of LivinαmRNA in urine exfoliated cells was significantly correlated with tumor The pathological grade and stage had no relation (P> 0.05). The sensitivity and Youden index of LivinαmRNA in urine exfoliated cells were significantly higher than that of urinary cytology (P <0.05), but the specificity of LivinαmRNA in urine exfoliated cells was not statistically significant (P> 0.05). Conclusion Detection of LivinαmRNA in exfoliated urothelial cells may be an ideal noninvasive diagnostic method for bladder urothelial carcinoma.