论文部分内容阅读
目的:观察脂质异常家兔抗氧化能力及血管细胞黏附因子-1(VCAM-1)的变化以及血脂康的干预。方法:采用高脂饲料喂养法复制血脂异常家兔模型,检测各组家兔血清胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、丙二醛(MDA)、超氧化物歧化酶(SOD)水平,并用酶联免疫吸附(ELISA)法测定家兔血清VCAM-1的含量。结果:高脂血症组家兔血清TC、TG、LDL-C、MDA、VCAM-1水平升高,SOD水平降低,与之相比,血脂康组家兔血清TC、TG、LDL-C、MDA水平降低(P<0.05),家兔血清中VCAM-1的含量降低(P<0.01),SOD水平明显升高(P<0.05)。结论:脂质异常可致家兔抗氧化能力降低,引起脂质过氧化,损伤内皮细胞,导致VCAM-1含量升高。血脂康可改善脂质异常家兔血清抗氧化能力、清除自由基、抗脂质过氧化损伤并下调VCAM-1的表达,提示其具有保护内皮细胞功能的作用。
OBJECTIVE: To observe the changes of antioxidant capacity and the expression of vascular cell adhesion molecule-1 (VCAM-1) in rabbits with dyslipidemia and the intervention of Xuezhikang. Methods: The model of dyslipidemia rabbits was duplicated by high-fat diet feeding. The levels of serum cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA) , Superoxide dismutase (SOD) were measured. Serum VCAM-1 levels were detected by enzyme linked immunosorbent assay (ELISA). Results: Serum levels of TC, TG, LDL-C, MDA and VCAM-1 in rabbits with hyperlipidemia were significantly higher than those in normal controls (P <0.05). The serum levels of VCAM-1 in rabbits decreased (P <0.01) and SOD levels increased significantly (P <0.05). Conclusion: Abnormal lipid levels can reduce the antioxidative capacity, lead to lipid peroxidation and damage the endothelial cells, resulting in the increase of VCAM-1 content. Xuezhikang can improve anti-oxidative ability, free radical scavenging, anti-lipid peroxidation injury and down-regulation of VCAM-1 expression in rabbits with lipid abnormalities, suggesting that it has the function of protecting endothelial cells.