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研究蚌肉多糖对四氯化碳诱导急性肝损伤小鼠的保护作用。将小鼠随机分为对照组、模型组、100 mg·kg-1蚌肉多糖组、200mg·kg-1蚌肉多糖组、护肝片组。实验组小鼠预防给药每天灌胃一次,连续8d。末次灌胃2h后,对照组腹腔注射调和油溶液,其他各组注射0.15%CCl4调和油溶液。测定血清ALT活性,肝匀浆NO含量和肝体指数,H&E染色观察肝脏组织形态学的改变。实验结果表明200mg·kg-1蚌肉多糖能显著抑制血清ALT活性和降低肝组织NO含量,组织形态学观察结果发现蚌肉多糖明显改善了肝细胞的水样变和脂肪变,阻遏炎性反应的发生,但对肝体指数无明显影响。因此,蚌肉多糖对四氯化碳诱导急性肝损伤小鼠有保护作用,作用机制可能与其抗炎作用有关。
To study the protective effect of mussel polysaccharides on carbon tetrachloride-induced acute liver injury in mice. The mice were randomly divided into control group, model group, 100 mg · kg-1 clam meat polysaccharide group, 200 mg · kg-1 clam meat polysaccharide group, Hugan tablet group. The mice in experimental group were given gavage once a day for 8 days. Two hours after the last gavage, the control group received intraperitoneal injection of reconstituted oil solution, and the other groups were injected with 0.15% CCl4 reconstituted oil solution. Serum ALT activity, liver homogenate NO content and hepatic body index were measured. H & E staining was used to observe the changes of liver histomorphology. Experimental results show that 200mg · kg-1 clam meat polysaccharide can significantly inhibit serum ALT activity and reduce the content of NO in liver tissue. Morphological observation showed that clam meat polysaccharide significantly improved the water-like hepatocytes and steatosis, inhibiting inflammatory response Of the occurrence, but no significant effect on liver index. Therefore, clam meat polysaccharide on carbon tetrachloride-induced acute liver injury in mice protective effect may be related to its anti-inflammatory effects.